Optimization of Laccase Extraction from Spent Pleurotus Substrate
نویسندگان
چکیده
منابع مشابه
Factorial design for optimization of laccase production from Pleurotus ostreatus IMI 395545 and laccase mediated synthetic dye decolorization
In the present study, Plackett–Burman design (PBD) followed by response surface methodology (RSM) was used to optimize the culture medium composition for laccase production from Pleurotus ostreatus IMI 395545. Influence of carbon, nitrogen (sources) and inducer were evaluated by Plackett–Burman design. The factors that induce positive effect on enzyme production were further optimized using cen...
متن کاملCharacteristics of Three Laccase Isoforms from the Basidiomycete Pleurotus nebrodensis
Figure S1. Cont. 68 PnLac1 95 PoLac 95 PsLac 95 PpLac 95 LsLac 65 HmLac . M M M M . . V V V V . . L L L L . . S S S S . . T T T T . . K K K K . . L L L L . . A A V V . . A A A A . . L L L L . . V V V V . . A A A A . . S S S S . . L L L L . . P P P P . . F F F F . . V V V V . . L L F F . . A A A A . . A A V V . . T T T T . . K K K K . . K K K K . . L L L L . . D D D D . . F F F F . . H H H H . ....
متن کاملIsolation, Purification, and Characterization of Fungal Laccase from Pleurotus sp.
Laccases are blue copper oxidases (E.C. 1.10.3.2 benzenediol: oxygen oxidoreductase) that catalyze the one-electron oxidation of phenolics, aromatic amines, and other electron-rich substrates with the concomitant reduction of O(2) to H(2)O. They are currently seen as highly interesting industrial enzymes because of their broad substrate specificity. A positive strain was isolated and characteri...
متن کاملThe white-rot fungus Pleurotus ostreatus secretes laccase isozymes with different substrate specificities.
Four laccase isozymes (LCC1, LCC2, LCC3 and LCC4) synthesized by Pleurotus ostreatus strain V-184 were purified and characterized. LCC1 and LCC2 have molecular masses of about 60 and 65 kDa and exhibited the same pI value (3.0). Their N termini were sequenced, revealing the same amino acid sequence and homology with laccases from other microorganisms. Laccases LCC3 and LCC4 were characterized b...
متن کاملHeterologous Expression of Laccase (LACP83) of Pleurotus ostreatus
The heterologous expression of the gene LACP83 (encoding a laccase) from Pleurotus ostreatus in Escherichia coli was characterized. The laccase enzyme activity and kinetics of bacterial growth with an inducer (IPTG) and without inducer were determined. The maximum enzymatic activity was observed at 7 h post induction with a value of 3740 ± 342 U/L, which was similar to that reported for the nat...
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ژورنال
عنوان ژورنال: Proceedings
سال: 2020
ISSN: 2504-3900
DOI: 10.3390/proceedings2020057077